期刊
BRAIN RESEARCH
卷 1134, 期 1, 页码 18-26出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2006.11.071
关键词
vasopressin; vasotocin; corticosterone; PKC; proteolysis; translocation
In urodele amphibians, the hypothalamic neuropeptide arginine vasotocin and the adrenal steroid corticosterone interact to regulate reproductive behavior by actions in the brain. The present study investigated signal transduction pathways underlying acute effects of vasotocin and corticosterone, presumably mediated via non-genomic steroid action, in an amphibian brain. We used Western blot to examine the effects of corticosterone and the vasotocin receptor agonist arginine vasopressin, alone and in combination, on the subcellular localization and proteolytic processing of protein kinase C-alpha (PKC alpha) in tiger salamander brain tissue. Treatment of whole brain minces with vasopressin or vasotocin led to increases in PKC alpha in membrane fractions and concurrent decreases in PKC alpha in cytosolic fractions. Vasopressin or vasotocin treatment also induced the appearance in membrane and cytosolic fractions of a PKC alpha-immunoreactive band that corresponds to PKM alpha, the proteolytically generated, free catalytic subunit of PKC alpha. Treatment with corticosterone alone had no consistent effect on either PKC alpha or PKM alpha in either fraction. However, pretreatment with corticosterone reliably blocked vasopressin-induced increases in cytosolic PKM alpha. These data provide new information about the cellular mechanisms of action of vasopressin and corticosterone in the vertebrate brain and suggest a cellular mechanism by which the two hormones interact to regulate neuronal physiology and behavior. (c) 2006 Elsevier B.V. All rights reserved.
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