期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 1, 页码 146-150出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201308141
关键词
artificial cells; cascade reactions; enzyme catalysis; nanoreactors; polymersomes
资金
- Dutch Science Foundation (NWO)
- European Science Foundation
- Ministry of Education, Culture and Science [024.001.035]
Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.
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