期刊
PLANT BIOTECHNOLOGY
卷 24, 期 2, 页码 217-220出版社
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
DOI: 10.5511/plantbiotechnology.24.217
关键词
Barley; grain filling; jasmonate; seed development; sugar import
资金
- Iwate University
- Iwate University 21st Century Center of Excellence Program Establishment of Thermobiosystem Research program, Japan
We previously showed the high expression of a 23 kD protein (P23k) in germinating barley seeds. In this study, we examined whether P23k is involved in supplying sugar to developing barley grains. RT-PCR analysis revealed that, although P23k expression was not detectable in developing grain, the 23 kD jasmonate-induced protein (JIP-23) exhibited a high level of RNA expression. JIP-23 belongs to the same protein family as P23k. Its remarkable expression was specifically confirmed at early development stage when sugar import into the filling grains is active. Furthermore, in situ hybridization and immunohistochemistry showed that JIP-23 is localized to active tissues in sugar import, such as vascular tissues, nucellar projections, endosperm transfer cells, and inter nucellar cells. Taken together, these results suggest that JIP-23 is related to sugar import into filling grain of developing barley seeds. Hence, we propose that jasmonate may regulate the development of barley seed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据