期刊
WATER RESEARCH
卷 41, 期 6, 页码 1169-1176出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2006.11.029
关键词
hepatitis A virus; Amazon Basin; genotype IA; genotype IB; qualitative PCR; quantitative real-time RT-PCR; environmental water samples
Hepatitis A virus (HAV) is a significant waterborne human pathogen. Of the global supply of potable water, Brazil retains 13%, of which 75% resides in the Amazon Basin. Although hepatitis A morbidity has declined progressively in Brazil as a whole, it remains high in the Amazon region. We used nested and real-time reverse-transcription polymerase chain reaction (RT-PCR) to detect and quantify the viral load in water samples from the Amazon Basin. Most samples tested positive (92%), with viral loads varying from 60 to 5500 copies/L, depending on sanitary conditions and the degree of flooding. Nested RT-PCR of the VP1-2A region detected HAV RNA in 23% of the samples. In low viral load samples, HAV was detected only with real-time RT-PCR, suggesting that this technique is useful for monitoring HAV contamination. The presence of HAV in water samples constitutes a serious public health problem. (c) 2007 Elsevier Ltd. All rights reserved.
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