期刊
BIOMEDICAL CHROMATOGRAPHY
卷 21, 期 3, 页码 284-290出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/bmc.754
关键词
antiarrhythmics; pharmacokinetics; amiodarone; LC/MS
A liquid chromatographic-mass spectrometry (LC/MS) assay method was developed for the determination of amiodarone and desethylamiodarone in rat specimens. Analytes were extracted using liquid-liquid extraction in hexane. The LC/MS system consisted of a Waters Micromass ZQ (TM) 4000 spectrometer with an autosampler and pump. A C-18 3.5 mu m (2.1 x 50 mm) column heated to 45 degrees C was used for separation. The mobile phase consisted of methanol and 0.2% aqueous formic acid pumped at 0.2 mL/min as a linear gradient. Components eluted within 12 min. The concentrations of ethopropazine (internal standard), desethylamiodarone and amiodarone were monitored for m/z of 313.10, combination of 546.9 and 617.73, and 645.83, respectively. In plasma (0.1 mL), linearity was achieved between the peak area ratios and concentrations over the range of 2.5-1000 ng/mL for both amiodarone and desethylamiodarone (r(2) > 0.999). The intraday and interday CV were equal or less than 18%, and mean error was < 12%. Similarly, in homogenates containing 0.1 of rat tissue, linearity was observed in standards ranging from 5 to 5000 ng/g. The method was successfully used to measure tissue and plasma concentrations of drug. The validated lower limit of quantitation was 2.5 ng/mL for drug and metabolite, based on 0.1 mL of plasma. Copyright (c) 2007 John Wiley & Sons, Ltd.
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