期刊
DEVELOPMENT
卷 134, 期 5, 页码 959-966出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.02775
关键词
pattern formation; cell-type specification; sporulation; encystation; adenylyl cyclase G; Dictyostelium discoideum
资金
- BBSRC [BB/D013453/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D013453/1] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/D013453/1] Funding Source: Medline
- Wellcome Trust [057137, 076618] Funding Source: Medline
Encystation and sporulation are crucial developmental transitions for solitary and social amoebae, respectively. Whereas little is known of encystation, sporulation requires both extra- and intracellular cAMP. After aggregation of social amoebae, extracellular cAMP binding to surface receptors and intracellular cAMP binding to cAMP-dependent protein kinase (PKA) act together to induce prespore differentiation. Later, a second episode of PKA activation triggers spore maturation. Adenylyl cyclase B (ACB) produces cAMP for maturation, but the cAMP source for prespore induction is unknown. We show that adenylyl cyclase G (ACG) protein is upregulated in prespore tissue after aggregation. acg null mutants show reduced prespore differentiation, which becomes very severe when ACB is also deleted. ACB is normally expressed in prestalk cells, but is upregulated in the prespore region of acg null structures. These data show that ACG induces prespore differentiation in wild-type cells, with ACB capable of partially taking over this function in its absence.
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