期刊
NATURE METHODS
卷 4, 期 3, 页码 215-217出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth1014
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资金
- NCRR NIH HHS [RR01219, P41 RR001219] Funding Source: Medline
Cryo-electron microscopy can provide high-resolution structural information about cells and organelles in the nearly native, frozen-hydrated state. Applicability, however, is limited by difficulties encountered in preparing suitably thin, vitreously frozen biological specimens. We demonstrate, by cryo-electron tomography of Escherichia coli cells, that a focused ion beam (FIB) can be used to thin whole frozen-hydrated cells in a convenient and essentially artifact-free way.
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