期刊
JOURNAL OF VIROLOGICAL METHODS
卷 140, 期 1-2, 页码 183-192出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2006.11.019
关键词
adeno-associoted virus; empty capsids; column chromatography
Adeno-associated virus (AAV) empty capsids typically co-purify with genome containing AAV2 vectors purified by Column chromatography. This study describes a method to remove empty capsids front genome containing vector particles by anion exchange chromatography. The separation is based on the slightly less anionic character of empty particles compared to vectors. Detailed methods to achieve AAV2 vector purification and particle separation using cation exchange resin POROS 50HS followed by anion exchange resin Q-Sepharose(x1) are described. Chromatographic separation of AAV2 particles was achieved using gradients based on sodium acetate and ammonium acetate, and was optinnal at pH 8.5. Efficient romoval of particle surface nucleic acid impurities was found to be important to achieve good particle, separation. In a large scale experiment performed using partially purified vector containing a Mixture of 1.56 x 10(14) vg and 2.52 x 10(15) empty capsids as a starting material, the optimized containing 0.25 x 10(14) empty capsids, corresponding to 74% anion exchange chrometography method resulted in a vector peak of 1.15 x 10(14) vg vector yield and 86-fold reduction in empty capsids in the vector product. (c) 2006 Elsevier B.V. All rights reserved.
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