期刊
VISION RESEARCH
卷 47, 期 5, 页码 624-633出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.visres.2006.11.020
关键词
mouse model; retinal degeneration; retinitis pigmentosa; rd1; rd10; nmf137; PDE6b; beta-phosphodiesterase; rod photoreceptor; cGMP-PDE; beta-subunit of rod cGMP phosphodiesterase gene
资金
- NEI NIH HHS [T32 EY007092, R01 EY007758, R01 EY014026, R24EY017045, P30 EY006360, R01 EY016470, P30 EY06360, R01 EY12514] Funding Source: Medline
We report the chromosomal localization, mutant gene identification, ophthalmic appearance, histology, and functional analysis of two new hereditary mouse models of retinal degeneration not having the Pde6b(r),(d1)(r, rd, or rodless) mutation. One strain harbors an autosomal recessive mutation that maps to mouse chromosome 5. Sequence analysis showed that the retinal degeneration is caused by a missense point mutation in exon 13 of the beta-subunit of the rod cGMP phosphodiesterase (beta-PDE) gene (Pde6b). The gene symbol for this strain was set as Pde6b(rd10), abbreviated rd10 hereafter. Mice homozygous for the rd10 mutation showed histological changes at postnatal day 16 (P16) of age and sclerotic retinal vessels at four weeks of age, consistent with retinal degeneration. Retinal sections were highly positive for TUNEL and activated caspase-3 immunoreactivity, specifically in the outer nuclear layer (ONL). ERGs were never normal, but rod and cone ERG a- and b-waves were easily measured at P18 and steadily declined over 90% by two months of age. Protein extracts from rd10 retinas were positive for P-PDE immunoreactivity starting at about the same time as wild-type (P10), though signal averaged less than 40% of wild-type. Interestingly, rearing rd10 mice in total darkness delayed degeneration for at least a week, after which morphological and functional loss progressed irregularly. With the second strain, a complementation test with rdl mice revealed that the retinal degeneration phenotype observed represents a possible new allele of Pde6b. Sequencing demonstrated a missense point mutation in exon 16 of the beta-subunit of rod phosphodiesterase gene, different from the point mutations in rdl and rd10. The gene symbol for this strain was set as Pde6b(nmf137), abbreviated nmf137 hereafter. Mice homozygous for this mutation showed retinal degeneration with a mottled retina and white retinal vessels at three weeks of age. The exon 13 missense mutation (rd10) is the first known occurrence of a second mutant allele spontaneously arising in the Pde6b gene in mice and may provide a model for studying the pathogenesis of autosomal recessive retinitis pigmentosa (arRP) in humans. It may also provide a better model for experimental pharmaceutical-based therapy for RP because of its later onset and milder retinal degeneration than rdl and nmf137. (c) 2006 Elsevier Ltd. All rights reserved.
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