Visible light and enzymatic induced synthesis of malic acid from pyruvic acid and HCO3- with the combination system of zinc chlorophyll derivative and malic enzyme in water media

Visible light and enzymatic induced synthesis of malic acid from pyruvic acid and HCO3- with malic enzyme (ME) and NADP(+) photoreduction by the visible light photosensitization of chlorophyll derivative, zinc chlorin-e(6) (Zn Chl-e(6)) in the presence of NADH as an electron-donating reagent was investigated. When the sample solution containing NADH, Zn Chl-e(6), methylviologen (MV2+), pyruvic acid, NaHCO3, NADP(+), ferredoxine-NADP(+)-reductase (FNR) and ME was irradiated with visible light (> 390 nm), malic acid was produced. The produced malic acid was 0.65 mmol dm(-3) after 3 h visible light irradiation. (c) 2006 Elsevier B.V. All rights reserved.