期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 34, 期 3, 页码 343-354出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2006.11.015
关键词
fragile X mental retardation protein; spines; dendritic mRNA; CPEB; staufen; BC1 RNA; FMR1 mRNA; alpha CaMKII mRNA
资金
- Telethon [GGP05269] Funding Source: Medline
The localization of RNA/mRNA in dendrites plays a role in both local and temporal regulation of protein synthesis, which is required for certain forms of synaptic plasticity. A key molecule in these processes is the fragile X mental retardation protein (FMRP). Using in situ hybridization coupled to immunofluorescence confocal microscopy, we find that the FMRP-RNP particle contains ceCaMKII and BC1 RNAs as well as Staufen and CPEB proteins. Furthermore, following mGluR activation, the FMRP-mRNP complex moves into spines as shown by co-localization with the PSD-95 and Shank proteins. This study shows, for the first time, that the translationally inactive FMRP-mRNP complex relocates into neuronal spines after stimulation and that de novo protein synthesis mainly contributes to the pool of FMRP at synapses. (c) 2006 Elsevier Inc. All rights reserved.
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