Oxidative status and serum PON1 activity in beta-thalassemia minor

Objectives: Paraoxonase-1 (PON1) deficiency is related to increased susceptibility to low density lipoprotein oxidation and development of atherosclerosis. The aim of this study was to investigate paraoxonase and arylesterase activities along with oxidative status parameters, and to find out if there is any increased susceptibility to atherogenesis, which might be reflected with increased oxidative stress and decreased serum PON1 activity in beta-thalassemia minor (BTM) subjects. Design and methods: Thirty-two subjects with BTM and 28 healthy subjects as control were enrolled in the study. Serum paraoxonase and arylesterase activities, lipid hydroperoxide (LOOH) levels, total antioxidant capacity (TAC), total oxidant status (TOS) and oxidative stress index (OSI) were determined. Results: Serum TAC, paraoxonase and arylesterase activities were significantly lower in BTM subjects than controls (for all p < 0.001), while TOS, LOOH levels and OSI were significantly higher (p < 0.001, p < 0.05 and p < 0.001; respectively). In BTM subjects, OSI, TOS, LOOH levels and TAC were significantly correlated with serum paraoxonase (r=-0.245, p < 0.05; r=-0.231, p < 0.05; r=-0.264, p < 0.05 and, r=0.342, p < 0.05, respectively) and arylesterase activities (r=-0.332, p < 0.05, r=-0.308, p < 0.05; r=0.320, p < 0.05 and r=0.443, p < 0.05). Additionally, hemoglobin level was also correlated with serum paraoxonase (r=0.501, p < 0.001) and arylesterase activities (r=0.501, p < 0.001), TAC (r=0.402, p < 0.05), TOS (r=-0.274, p < 0.05) and OSI (r=-0.352, p < 0.05). Conclusions: Oxidative stress is increased, while serum PON1 activity is decreased in BTM subjects. Decrease in PON1 activity seems to be associated with both the degree of oxidative stress and anemia. BTM subjects may be more prone to development of atherogenesis due to low serum PON1 activity. (c) 2007 Published by The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.