期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 68, 期 3, 页码 605-612出版社
ELSEVIER
DOI: 10.1016/j.mimet.2006.11.010
关键词
bacteria; adhesion and biofilm formation ability; screening device; BioFilm Ring Test (R)
This work describes the implementation of a new assay named the BioFilm Ring Test (R) to evaluate the ability of bacteria to form biofilms. This assay is based on the immobilisation (or not) of magnetic beads embedded by bacterial aggregates or mats (pattented concept). It is realised on modified polystyrene 96-wells microtiter plates with individual 8-wells slides. The kinetic of bicifilm formation of four bacterial species, Listeria monocytogenes. Escherichia coli, Staphylococcus carnosus and Staphylococcus xylosus was evaluated with this new device by comparison with the standard crystal violet staining method of sessile cells. In parallel, the biofilm growth was visualized by Scanning Electron Microscopy (SEM) observations. The BioFilm Ring Test (R) gave similar but faster results than the crystal violet method. Moreover, the new assay was easier to implement.. more reproducible and allowed high throughput screenings due to limited manipulations (no washing and staining steps) and rapid and accurate measurements of magnetic bead immobilisation by sessile bacterial cells. (c) 2006 Elsevier B.V. All rights reserved.
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