期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 14, 期 3, 页码 246-248出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb1206
关键词
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资金
- NIGMS NIH HHS [GM33289, R01 GM033289] Funding Source: Medline
Myosin V moves cargoes along actin filaments by walking hand over hand. Although numerous studies support the basic hand-over-hand model, little is known about the fleeting intermediate that occurs when the rear head detaches from the filament. Here we use submillisecond dark-field imaging of gold nanoparticle-labeled myosin V to directly observe the free head as it releases from the actin filament, diffuses forward and rebinds. We find that the unbound head rotates freely about the lever-arm junction, a trait that likely facilitates travel through crowded actin meshworks.
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