期刊
ELECTROANALYSIS
卷 19, 期 5, 页码 604-607出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200603769
关键词
NADH; Ti-MCM-41; electrocatalytic; biosensors; dehydrogenases
Titanium-containing MCM-41 (Ti-MCM-41) modified glassy carbon electrode (GCE) can exhibit an excellent electrocatalytic activity towards the oxidation of beta-Nicotinamide adenine dinucleotide (NADH). A dramatic decrease in the over-voltage of NADH oxidation reaction is observed at 0.28 V (vs. SCE). The modified electrode is found to be stable and reproducible. The electrode shows a linear response for a wide range of 10 - 1200 mu M NADH and the detection limit is 8.0 mu M. Ti-MCM-41 mesoporous molecular sieves provide an efficient matrix for development of NADH biosensors and the prepared electrode not only can be used to detect the concentration of NADH in biochemical reaction, but also as the potential matrix of the construction of dehydrogenases biosensor.
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