期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 387, 期 5, 页码 1939-1944出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-1100-3
关键词
MALDI-MS; binary matrix; matrix background suppression; small molecule detection
Application of matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) to small-molecule detection is often limited, because of high matrix background signals in the low-mass region. We report here an approach in which a mixture of two conventional MALDI matrices with different proton affinity was used to suppress the formation of matrix clusters and fragments. Specifically, when acidic alpha-cyano-4-hydroxycinnamic acid (CHCA) and basic 9-aminoacridine (9-AA) were used as the binary matrix, fewer background matrix peaks were observed in both positive and negative-mode detection of small molecules. In addition, the presence of CHCA substantially reduced the laser fluence needed for analyte desorption and ionization; thus better signal-to-background ratios were observed for negatively charged inositol phosphates in complex plant extracts.
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