First-order conformation transition of single poly(2-vinylpyridine) molecules in aqueous solutions

By measuring diffusion rate, the conformation change of single poly(2-vinylpyridine) chain in aqueous solution was studied by fluorescence correlation spectroscopy. The data showed a stepwise change of hydrodynamic radius when pH value was tuned, reflecting a sign of first-order conformation transition, and a continuous change was found at varying salt concentration. (c) 2007 American Institute of Physics.