期刊
VETERINARY MICROBIOLOGY
卷 120, 期 3-4, 页码 328-333出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2006.11.001
关键词
actin; intestinal epithelium; time course; neural Wiskott-Aldrich syndrome protein; Rho GTPase
资金
- NIDA NIH HHS [R01 DA010200, R01 DA-10200] Funding Source: Medline
Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Racl GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Racl GTPase-independent changes in epithelial actin assembly. (c) 2006 Elsevier B.V. All rights reserved.
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