Combined antioxidant effects of rutin and vitamin C in Triton X-100 micelles

UV-vis spectra, fluorescence emission spectra and cyclic voltammetric measurements were used to study the influence of Vitamin C on the antioxidant of rutin in Triton X- 100 micelles. Rutin can be located in Triton X- 100 micelles spontaneously through hydrophobic force, and the binding constant K between rutin and Triton X- 100 increases with the rutin concentration. The embedment of two hydroxyl groups on rutin into the more hydrophobic micellar microenvironment makes the oxidation of rutin harder and the radical scavenging activity decrease. With low concentration of Vitamin C, the antioxidant capacity of rutin against hydroxyl radical is enhanced, while that capacity is partly inhibited when the concentration of Vitamin C become higher. (c) 2006 Elsevier B.V. All rights reserved.