期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 8, 页码 1672-1679出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.07.027
关键词
microbial fuel cell; Shewanella oneidensis; DSP10; mini-MFC; biofilm
A miniature-microbial fuel cell (mini-MFC, chamber volume: 1.2 mL) was used to monitor biofilm development from a pure culture of Shewanella oneidensis DSP10 on graphite felt (GF) under minimal nutrient conditions. ESEM evidence of biofilm formation on GF is supported by substantial power density (per device cross-section) from the mini-MFC when using an acellular minimal media anolyte (1500 mW/m(2)) . These experiments demonstrate that power density per volume for a biofilm flow reactor MFC should be calculated using the anode chamber volume alone (250 W/m(3)), rather than with the full anolyte volume. Two oxygen reduction cathodes (uncoated GF or a Pt/vulcanized carbon coating on GF) were also compared to a cathode using uncoated GF and a 50 mM ferricyanide catholyte solution. The Pt/C-GF (2-4% Pt by mass) electrodes with liquid cultures of DSPIO produced one order of magnitude larger power density (150 W/m(3)) than bare graphite felt (12 W/m(3)) in this design. These advances are some of the required modifications to enable the mini-MFC to be used in real-time, long-term environmental power generating situations. (c) 2006 Elsevier B.V. All rights reserved.
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