期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 8, 页码 1816-1819出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.08.008
关键词
charge-transfer resistance; single-base mismatch; DNA sensor; duplex; electrochemical impedance spectroscopy; single-nucleotide polymorphisms
Herein, we report an anomalous electrochemical behavior of surface-bound DNA duplex that has single-base mismatches at its distal end. Single-stranded 15-base DNA was immobilized at its 5'end onto gold electrode surfaces. After hybridization with complementary or mismatched DNA, electrochemical impedance spectra were obtained using [Fe(CN)(6)](3-/4-) as redox marker ions. Hybridization with the complementary DNA reduced the charge-transfer resistance (R-CT), whereas single-base mismatches at the distal end of the duplex largely increased the R-CT. This anomaly was found only with the distal end: the increase in R-CT was not observed for mismatches at either the middle or the proximal end. These results indicate that electrochemical detection of single-base alterations at an end of sample DNA is exceptionally easy because of the diametrically opposite responses. This detection principle is promising for the typing of single-nucleotide polymorphisms in combination with the single-base primer extension protocol. (c) 2006 Elsevier B.V. All rights reserved.
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