Study on DNA damage induced by CdSe quantum dots using nucleic acid molecular light switches as probe

The calf thymus DNA (ctDNA) damage induced by water-soluble CdSe quantum dots (QDs) was investigated using nucleic acid molecular light switches as probe. It was found that little ctDNA was damaged by CdSe QDs without UV irradiation. However, under UV irradiation, ctDNA was nicked by CdSe QDs very clearly. The mechanism of ctDNA damage was also discussed. The results strongly suggested that the ctDNA damage caused by CdSe QDs was not due to photo-induced liberation of Cd2+, but due to the production of free radicals and reactive oxygen species. (c) 2006 Elsevier B.V. All rights reserved.