期刊
JOURNAL OF IMMUNOLOGY
卷 178, 期 6, 页码 3620-3626出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.178.6.3620
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gamma delta T cells are present in the mucosal intestinal epithelia and secrete factors necessary to maintain tissue integrity. Ags recognized by these cells are poorly defined, although in mice non-classical MHC class I molecules have been implicated. Since MHC class I-like CD1 receptors are widely expressed at the surface of epithelial and dendritic intestinal cells and have the capacity to present lipid Ags to T cells, we hypothesized that these molecules might present autologous and/or exogenous phospholipids to intestinal gamma delta T lymphocytes. Intraepithelial T lymphocytes from normal human duodenal mucosal biopsies were cloned and exposed to natural and synthetic phospholipids using CD1a-, CD1b-, CD1c- or CD1d-transfected C1R lymphoblastoid or HeLa cell lines as APCs. Their cytolytic properties and regulatory cytokine secretion were also examined. Most clones obtained from duodenal mucosa (up to 70%) were TCR alpha beta(+), and either CD4(+) or CD8(+), whereas 20% were CD4(-)CD8(-) (6 clones) or TCR gamma delta(+) (12 clones). A relevant percentage (up to 66%) of TCR gamma delta(+) but few (< 5%) TCR alpha beta(+) T cell clones responded to synthetic and/or natural phospholipids presented by CD1 molecules, as measured by both [H-3]thymidine incorporation and IL-4 release assays. A Th1-like cytolytic and functional activity along with the ability to secrete regulatory cytokines was observed in most phospholipid-specific gamma delta T cell clones. Thus, a substantial percentage of TCR gamma delta(+) but few TCR alpha beta(+) from human duodenal mucosa recognize exogenous phospholipids in a CD1-restricted fashion. This adaptive response could contribute to mucosal homeostasis, but could also favor the emergence of inflammatory or allergic intestinal diseases.
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