期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 72, 期 6, 页码 2088-2096出版社
AMER CHEMICAL SOC
DOI: 10.1021/jo062341m
关键词
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资金
- National Research Foundation of Korea [R17-2007-017-01001-0, R16-1998-011-01001-0, 2004-201-C00067, 과C6A2204] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (K-d(i)) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).
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