期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 129, 期 12, 页码 3722-3728出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja067159h
关键词
-
资金
- NCRR NIH HHS [S10 RR017948, 1S10RR017948] Funding Source: Medline
- NIAID NIH HHS [1 F32 AI068605-0, F32 AI068605] Funding Source: Medline
- NIGMS NIH HHS [R01 GM030367, R01 GM030367-25, R37 GM030367, GM30367] Funding Source: Medline
This paper describes the synthesis of the trivalent hapten molecule 1, containing three 2,4-dinitrophenyl (2,4-DNP) groups, and the use of this molecule to aggregate three molecules of anti-2,4-DNP IgG into a complex with 3:2 stoichiometry (IgG(3)1(2)). The equilibrium product IgG(3)1(2) was generated in similar to 90% yield upon mixing IgG and 1; during incubation, thermodynamically unstable, high-molecular-weight aggregates (> 10(4) nm in diameter) form first and convert subsequently to IgG(3)1(2). The thermodynamics and the kinetics of the formation of aggregates were studied using size-exclusion high-performance liquid chromatography (SE-HPLC), dynamic light scattering (DLS), and analytical ultracentrifugation (AUC). An analytical model based on multiple species in equilibrium was developed and used to interpret the SE-HPLC data. The aggregate IgG(3)1(2) was more stable thermodynamically and kinetically than monomeric aggregates of this IgG with monomeric derivatives of 2,4-DNP; this stability suggests potential applications of these aggregates in biotechnology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据