期刊
SCIENCE
卷 315, 期 5820, 页码 1817-1822出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1136782
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资金
- NCI NIH HHS [5T32 CA101968] Funding Source: Medline
- NHLBI NIH HHS [HL40387, P01 HL040387, HL54500] Funding Source: Medline
- NIGMS NIH HHS [5T32 GM08275, T32 GM008275, GM60610, GM54616] Funding Source: Medline
A variety of methods exist for the design or selection of antibodies and other proteins that recognize the water-soluble regions of proteins; however, companion methods for targeting transmembrane (TM) regions are not available. Here, we describe a method for the computational design of peptides that target TM helices in a sequence-specific manner. To illustrate the method, peptides were designed that specifically recognize the TM helices of two closely related integrins (alpha(IIb)beta(3) and alpha(v)beta(3)) in micelles, bacterial membranes, and mammalian cells. These data show that sequence-specific recognition of helices in TM proteins can be achieved through optimization of the geometric complementarity of the target-host complex.
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