期刊
BRAIN RESEARCH
卷 1139, 期 -, 页码 60-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2006.12.055
关键词
prion; Prnp; expression regulation; transgenic mice; beta-galactosidase
资金
- NIA NIH HHS [P01 AG002132, P01 AG021601-03, P01 AG010770, P01 AG010770-13, P01 AG021601, AG021601, P01 AG002132-26, AG02132, AG10770] Funding Source: Medline
Since prion protein (PrP) mRNA and PrPC expression levels in transgenic (Tg) mice using the CosSHa.tet vector correlate well with the PrP transgene copy, we constructed Prnp-LacZ Tg animals expressing beta-galactosidase that was inserted into the CosSHa.tet vector. The CosSHa.tet vector was created from a large PrP cosmid clone in which the PrP open reading frame was deleted. In the developing nervous system, the beta-galactosidase marker was not expressed in the neural progenitors of the mitotically active ventricular zone. It is first expressed in cells that have ceased proliferating, migrated radially from the ventricular zone, and differentiated into neurons in the intermediate layer. At E11.5 p.c., motor neurons in the ventral neural tube clearly express the marker transgene. Expression in dorsal neural tube neurons is observed at later stages, after their differentiation. These results indicate that Prnp gene expression in the nervous system begins in post-mitotic neural cells that have undergone neuronal differentiation. This pattern of Prnp expression in the nervous system appears to persist throughout the adult life of mammals. (c) 2007 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据