期刊
VIROLOGY
卷 360, 期 1, 页码 1-5出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2006.12.022
关键词
integrase; full-site integration; HIV-1; LEDGF/p75
类别
资金
- NIAID NIH HHS [AI60354, AI70042, P30 AI060354, R37 AI039394, R01 AI039394, R01 AI070042, AI39394] Funding Source: Medline
An integrase dimer can process and integrate a single HIV-1 DNA end in vitro, whereas a tetramer is required to integrate two ends. LEDGF/p75 can potently stimulate integrase activity, but its effects on half- versus full-site integration have not been investigated. Stimulation of half-site but inhibition of full-site integration is revealed here. LEDGF/p75 seems to interfere with integrase oligomerization, but does not inhibit the catalytic activity of pre-assembled complexes. We therefore speculate that LEDGF/p75 function is restricted to a point in the viral lifecycle that occurs after the formation of the preintegration synaptic complex, for example, as a chroinatin-associated tethering factor. (c) 2006 Elsevier Inc. All rights reserved.
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