期刊
NATURE METHODS
卷 4, 期 4, 页码 323-326出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1033
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资金
- NEI NIH HHS [F32 EY015063] Funding Source: Medline
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
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