期刊
NATURE IMMUNOLOGY
卷 8, 期 4, 页码 409-418出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ni1442
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- NCI NIH HHS [P01 CA71932, CA48737] Funding Source: Medline
- NIAID NIH HHS [AI069259, AI061663] Funding Source: Medline
- NIAMS NIH HHS [AR42689] Funding Source: Medline
- NIDDK NIH HHS [DK48247] Funding Source: Medline
Lymphocyte homing is mediated by specific interaction between L-selectin on lymphocytes and the carbohydrate ligand 6-sulfo sialyl Lewis X on high endothelial venules. Here we generated mice lacking both core 1 extension and core 2 branching enzymes to assess the functions of O-glycan-borne L-selectin ligands in vivo. Mutant mice maintained robust lymphocyte homing, yet they lacked O-glycan L-selectin ligands. Biochemical analyses identified a class of N-glycans bearing the 6-sulfo sialyl Lewis X L-selectin ligand in high endothelial venules. These N-glycans supported the binding of L-selectin to high endothelial venules in vitro and contributed in vivo to O-glycan-independent lymphocyte homing in wild-type and mutant mice. Our results demonstrate the critical function of N-glycan-linked 6-sulfo sialyl Lewis X in L-selectin-dependent lymphocyte homing and recruitment.
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