Tamlana crocina gen nov, sp nov, a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea

A Gram-negative, aerobic, saffron-coloured marine bacterium, designated HST1-43, was isolated from beach sediment on the coast in Jeju, Korea, and its taxonomic status was established in a polyphasic study. 16S rRNA gene sequence analyses revealed that the isolate belonged to the family Flavobacteriaceae. The closest phylogenetic neighbours of strain HST1-43(T) were members of the genera Algibacter, Gaetbulibacter and Yeosuana, with levels of sequence similarity in the range 96.3-96.5%. The isolate was non-motile and required sea salts or natural seawater for growth. The optimum temperature and pH ranges for growth were 25-30 degrees C and pH 6.1-8.1, respectively. MK-6 was the major menaquinone. The dominant cellular fatty acids were iso-C-15:0, C-18:0, C-16:0, iSO-C-15:1 and iso-C-17:0 3-OH. The DNA G + C content was 36.2 +/- 0.4 mol%. On the basis of phylogenetic distance and phenotypic characteristics, the isolate is considered to represent a novel genus and species in the family Flavobacteriaceae, for which the name Tamlana crocina gen. nov., sp. nov. is proposed. The type strain is HST1-43(T) (= KCTC 1272(T) = JCM 14021(T)).