期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 17, 期 7, 页码 1912-1915出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2007.01.040
关键词
cytosine methylcytosine; bisulfite; epigenotyping; hydrolysis; DNA
A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA. (c) 2007 Elsevier Ltd. All rights reserved.
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