The effects of Fusilade (Fluazifop-p-butyl) on germination, mitotic frequency and α-amylase activity of lentil (Lens culinaris Medik.) seeds

In this study, seed germination percentages, effects on phases of mitosis and alpha-amylase enzyme activity of lentil seeds treated with four different concentrations (0.25, 0.5, 1 and 1.5%) of Fusilade (Fluazifop-p-butyl) were determined. Median EC ( effective concentration) values were calculated according to seed germination percentages after treatment for 72 h. Germination percentages of primary lentil roots decreased with increasing Fusilade concentrations. Cytological observations showed that the mitotic frequency in root meristematic cells were decreased parallel to the increase in concentrations and all Fusilade concentrations applied decreased the activity of a-amylase enzyme in lentil seeds. The obtained results indicate that the herbicide Fusilade had the ability to cause reduction in seed germination, mitotic frequency and also alpha-amylase activity of lentil seeds.