期刊
MICROFLUIDICS AND NANOFLUIDICS
卷 3, 期 2, 页码 189-194出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10404-006-0119-2
关键词
SU-8; microfluidics; electrophoretic micromanipulation; rapid prototyping
We describe the fabrication and application of a bioanalytical chip, made of SU-8 photoresist, comprising integrated, high aspect-ratio microfluidic channels, suitable to manipulate and investigate vesicles, cell fragments and biological cells. A central micrometer-sized aperture allows electrical particle counting and planar membrane experiments, microfluids allow (sub)micrometer-sized objects to be transported and addressed with different chemicals. Here we show how lipid vesicles are positioned with micrometer precision within the micro-channels by means of pressure and electrophoretic movement. Our approach is suited for controlling and investigating (bio)chemical synthesis and cellular signalling processes in ultrasmall individual vesicles by electro-optical techniques.
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