期刊
BIOMATERIALS
卷 28, 期 12, 页码 2087-2096出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2006.12.028
关键词
BMP; controlled delivery; nano fiber; scaffold; tissue engineering; nanospheres
资金
- NIDCR NIH HHS [R21 DE014755-01A1, R01 DE015384, R01 DE015384-02, R01 DE015384-01, DE15384, R01 DE013397, R21 DE014755, DE14755, R21 DE014755-02, R01 DE013397-05, DE13397, R01 DE015384-03, R01 DE013397-04, R01 DE015384-04] Funding Source: Medline
It is advantageous to incorporate controlled growth factor delivery into tissue engineering strategies. The objective of this study was to develop a three-dimensional (313) porous tissue engineering scaffold with the capability of controlled releasing recombinant human bone morphogenctic protein-7 (rhBMP-7) for enhariccment of bone regeneration. RhBMP-7 was first encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanospheres (NS) with an average diameter of 300nm. Poly(L -lactic acid) (PLLA) scaffolds with interconnected macroporous and nano-fibrous architectures were prepared using a combined sugar sphere template leaching and phase separation technique. A post-seeding technique was then utilized to immobilize rhBMP-7 containing PLGA nanospheres onto prefabricated nanofibrous PLLA scaffolds with well-maintained 3D, structures. In vitro release kinetics indicated that nanosphere immobilized scaffold (NS-scaffold) could release rhBMP-7 in a temporally controlled manner, depending on the chemical and degradation properties of the NS which were immobilized onto the scaffold. In vivo, rhBMP-7 delivered from NS-scaffolds induced significant ectopic bone formation throughout the scaffold while passive adsorption of rhBMP-7 into the scaffold resulted in failure of bone induction due to either the loss of rhBMP-7 biological function or insufficient duration within the scaffold. We conclude that the interconnected macroporous architecture and the sustained, prolonged delivery of bioactive rhBMP-7 from NS immobilized nano-fibrous scaffolds actively induced new bone formation throughout the scaffold. The approach offers a new delivery method of BMPs and a novel scaffold design for bone regeneration. (c) 2007 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据