期刊
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
卷 30, 期 4, 页码 279-284出版社
EDITRICE KURTIS S R L
DOI: 10.1007/BF03346294
关键词
Rhes; G-protein coupled receptors; cAMP; PKA
Ras homolog enriched in striatum (Rhes) is a member of the Ras family of small GTPases detected in the thyroid. Rhes inhibits signal transcluction from G alpha s protein. In this study we investigated whether Rhes can interfere with stimulation of cAMP/protein kinase A (PKA) pathway of TSH, FSH and LH receptors (TSHr, FSHr, LHr) and of activated TSHr mutants. Receptors were transiently transfected in COS-7 cells with or without Rhes; cAMP was evaluated in basal conditions and after hormone stimulation. Constitutive and bovine TSH (bTSH)-stimulated activity of wild type (wt) and mutated TSHr was inhibited after Rhes co-transfection. Rhes decreased cAMP after FSH and hCG P-subunit (PhCG) stimulation in cells expressing the cognate receptors. In binding experiments Rhes, as another membrane protein, sodium/iodide symporter (NIS), reduced membrane expression of wt TSHr (wtTSHr). In conclusion, Rhes can interfere with the functional activity of wt and mutated TSHr and with the respective hormone-stimulated cAMP production of FSHr and LHr. This interference is not specific and due to the co-expression of two membrane proteins.
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