期刊
JOURNAL OF SEPARATION SCIENCE
卷 30, 期 6, 页码 858-867出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200600466
关键词
characterization; flavanone aglycones; Fructus aurantii; LC-MSIMS; quantification
IT-MS operated in the positive mode Was applied for the rapid characterization/ quantification of the flavanones in extracts from Fructus aurantii. APCI-MS and CID MS/MS provide unequivocal molecular weight (MW) data of these compounds and useful information about their structures (diagnostic fragment ions). Main fragment pathways include neutral losses of H,O-2, C2H2O, and B-ring as well as a retro-Diels-Alder (RDA) fragment giving rise to [(1,3)A + H](+), [B-1,B-3 + H](+), and [B-1,B-4-H-2 + H](+) ions, which form the characteristic MS/MS fingerprint of flavanone aglycones. When screening extracts of F. aurantii for flavanone aglycones, eight target compounds were characterized using this fingerprint. Meanwhile, ESI-MS in full-scan mode was developed and validated for the quantification of the main flavanone aglycones in F. aurantii. This method is simple, accurate, fast and requires only 16 min per sample for direct detection and quantification of naringenin and hesperetin. All the results and these characteristic fragments showed that the IT-MS is a powerful tool for the structural characterization and quantitative determination of flavanone aglycones.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据