Direct electrochemical conversion of bilirubin oxidase at carbon nanotube-modified glassy carbon electrodes

We report on direct electron transfer reactions of bilirubin oxidase at multi-walled carbon nanotube (MWCNT) modified glassy carbon electrodes (GCE). The bioelectrocatalytic oxygen reduction was recorded using linear sweep voltammetry (LSV) with BOD in solution, adsorbed and covalently linked to the nanotubes. The MWCNT modification of GC electrodes strongly enhances the oxygen reduction compared to the signals at unmodified GCE. Under anaerobic conditions with a high protein concentration in solution a pair of redox peaks with a formal potential of 450 +/- 15 mV vs Ag/AgCl, 1 M KCl (pH 7.4) was found with cyclic voltammetry. The redox conversion is indicated to be surface-controlled and pH-dependent (54.5 mV/pH). The quasi-reversible redox reaction might be attributed to the trinuclear T2/T3 cluster of BOD. (c) 2006 Published by Elsevier B.V.