期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 73, 期 7, 页码 2324-2328出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02038-06
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资金
- NCI NIH HHS [CA112418, R21 CA112418] Funding Source: Medline
An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.
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