期刊
INFLAMMATION RESEARCH
卷 56, 期 4, 页码 143-148出版社
BIRKHAUSER VERLAG AG
DOI: 10.1007/s00011-006-6136-5
关键词
heat shock protein 65 (hsp65); inflammatory bowel disease; microparticles; particulate antigen
资金
- Medical Research Council [MC_U105960399, G0000135] Funding Source: researchfish
- MRC [G0000135, MC_U105960399] Funding Source: UKRI
- Medical Research Council [MC_U105960399, G0000135] Funding Source: Medline
Objectives: The aims of this study were to determine, in peripheral blood mononuclear cells (PBMC), whether particulate antigen triggers (i) an amplified cell proliferative response compared to soluble antigen and (ii) a dysfunctional response in cells derived from patients with chronic inflammation and specifically in those with inflammatory bowel disease (IBD). Subjects: Healthy volunteers (n = 17), inflammatory controls (n = 8) and patients with IBD (n = 17) were recruited from St Thomas' and Guys' Hospital, London, UK. Methods: Following optimisation of experimental conditions (0.1-10.0 mu g/ml antigen), PBMC were stimulated with (i) 10.0 mu g/ml recombinant soluble heat shock protein 65 (hsp 65) and (ii) 1.0 and 10.0 mu g/ml hsp 65 conjugated to microparticles (0.5 mu m diameter). PBMC proliferative responses were measured by H-3-Thymidine incorporation at day 5 and results compared between groups using unpaired t-test. Results: Conjugation to microparticles of low dose hsp 65 significantly increased overall proliferative responses by 2-11 fold compared to soluble antigen alone (p < 0.05). However, no specific PBMC proliferative dysregulation was noted in cells from subjects with IBD. Conclusions: Low dose antigen, in microparticulate form, leads to amplified cell proliferation in primary human cells, as showed previously in cell lines and animal studies. However there is no abnormal proliferative response in cells from subjects with IBD.
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