期刊
ENVIRONMENTAL MICROBIOLOGY
卷 9, 期 4, 页码 971-982出版社
WILEY
DOI: 10.1111/j.1462-2920.2006.01219.x
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资金
- NERC [pml010003, pml010004, pml010001, NE/D001455/1, mba010002] Funding Source: UKRI
- Natural Environment Research Council [pml010004, mba010002, pml010003, pml010001, NE/D001455/1] Funding Source: researchfish
The diversity among the coccolithovirus strains held in the Plymouth Virus Collection (PVC) was assessed using three complementary techniques: phylogeny based on DNA polymerase and major capsid protein gene sequence; host range; and a new, microarray-based genome-wide approach. The PVC is composed of three groups of strains that are geographically and temporally distinct. Virus strains clustered according to these groups in all three diversity assessments. Furthermore, the microarray approach based on genomic content showed that two strains, previously considered as identical to others in the PVC, are actually distinct. These results show the importance of genome-wide surveys for assessing strain diversity. Not only has the microarray provided an alternative to the phylogeny-derived pattern for virus evolution, it has also begun to provide some clues to the genes that may be responsible for the different phenotypes displayed by these viruses.
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