Rapid determination of volatile compounds in Toona sinensis (A. Juss.) Roem. by MAE-HS-SPME followed by GC-MS

Toona sinensis (A. Juss.) Roem. belonging to the Toona family was used as one of the traditional Chinese medicines in China. Based on microwave-assisted extraction (MAE) and headspace solid-phase microextraction followed by gas chromatography-mass spectrometry, the volatile compounds of Toono sinensis (A. Juss.) Roem. were determined. Results indicated that the optimum condition of the determination of the volatile compounds in Toona sinensis (A. Juss.) Roem. was achieved with the experimental parameters including fiber coating of PDMS/DVB, microwave power of 400 W and irradiation time of 4 min. Under the optimal conditions, for the first time, 45 volatile compounds were separated and identified from the fresh leaves of Toona sinensis (A. Juss.) Roem. The highest content component of the 45 compounds was trans-Caryophyllene (21.422%). Relative standard deviation (RSD) values < 9% showed that the present method has good precision. The experimental results demonstrate that MAE-HS-SPME followed by GC-MS is a simple, time-saving and solvent-free method, and it is a potential analytic tool for the determination of the volatile compounds of TCMs.