期刊
JOURNAL OF MICROSCOPY
卷 226, 期 1, 页码 33-42出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2007.01751.x
关键词
adaptive optics; live imaging aberrations; microscopy
类别
Wide-field fluorescence microscopy is an essential tool in modern cell biology. Unfortunately the image quality of fluorescence microscopes is often significantly degraded due to aberrations that occur under normal imaging conditions. In this article, we examine the use of adaptive optics technology to dynamically correct these problems to achieve close to ideal diffraction limited performance. Simultaneously, this technology also allows ultra-rapid focusing without having to move either the stage or the objective lens. We perform optical simulations to demonstrate the degree of correction that can be achieved.
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