期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 848, 期 2, 页码 182-187出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2006.10.017
关键词
resveratrol; conjugated metabolites; HPLC; human; plasma; urine
资金
- NCI NIH HHS [N01-CN-25025-3, N01 CN025025] Funding Source: Medline
We describe a reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify six major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of I g. Waters Atlantis C-18 3 mu m served as the stationary phase. The gradient was formed using ammonium acetate and methanol, containing 2% propan-2-ol. Detection is linear between 5 ng/mL and 500 ng/mL in plasma (5-1000 ng/mL in urine). The coefficient of variation for intra- and inter-day variation is <10%. The average recovery of resveratrol from plasma and urine is 58 +/- 3%. The data presented in this report demonstrate a rapid, sensitive and accurate method for the analysis of resveratrol and its metabolites in human plasma and urine for pharmacokinetic studies. (c) 2006 Elsevier B.V. All rights reserved.
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