期刊
CLINICA CHIMICA ACTA
卷 379, 期 1-2, 页码 127-133出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2006.12.029
关键词
global hypomethylation; prognostic marker; hepatocellular carcinoma; COBRA LINE-1; hepatoma
Background: We investigated the clinical implications of global hypomethylation, one of the most consistent epigenetic changes in cancer, in the sera of patients with hepatocellular carcinoma (HCC). Methods: Combined bisulfite restriction analysis PCR was used to assess the methylation status of LINE-1 repetitive sequences in genomic DNA derived from sera of 85 patients with HCC, 73 patients with cirrhosis, 20 healthy carriers of hepatitis B virus (HBV) and 30 healthy controls. Results: Serum genome hypomethylation, the percentage of unmethylated LINE-1, was significantly increased in patients with HCC (P<0.001). The levels of serum LINE-1 hypomethylation at initial presentation correlated significantly with the presence of HBsAg, large tumor sizes, and advanced tumor stages classified by the CLIP score. Multivariate analyses showed that serum LINE-1 hypomethylation was a significant and independent prognostic factor of overall survival. Conclusion: Serum LINE-1 hypomethylation may serve as a prognostic marker for patients with HCC. (c) 2007 Elsevier B.V. All rights reserved.
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