期刊
FEMS MICROBIOLOGY LETTERS
卷 269, 期 1, 页码 63-69出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2006.00605.x
关键词
Flavobacterium columnare; SSCP; 16S rRNA gene; ISR; genotyping
类别
Flavobacterium columnare intraspecies diversity was revealed by analyzing the 16S rRNA gene and the 16S-23S internal spacer region (ISR). Standard restriction fragment length polymorphism (RFLP) of these sequences was compared with single-strand conformation polymorphism (SSCP). Diversity indexes showed that both 16S-SSCP and ISR-SSCP improved resolution (D >= 0.9) when compared with standard RFLP. ISR-SSCP offered a simpler banding pattern than 16S-SSCP while providing high discrimination between isolates. SSCP analysis of rRNA genes proved to be a simple, rapid, and cost-effective method for routine fingerprinting of F. columnare.
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