期刊
NATURE METHODS
卷 4, 期 4, 页码 319-321出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1024
关键词
-
资金
- NINDS NIH HHS [R01 NS035549-12, R01 NS035549-11, R01 NS035549] Funding Source: Medline
The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据