期刊
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
卷 71, 期 4, 页码 547-552出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.ijporl.2006.11.025
关键词
tissue engineered cartilage; microtia reconstruction; external ear malformations
Objective: One current technique to reconstruct an ear for microtia involves the use of a high density polyethylene auricular implant; however, the implant can extrude if not covered in a temporoparietal fascia flap. Theoretically, an autologous tissue engineered cartilage bioshell protective coating around a permanent biocompatible implant might reduce potential extrusion to avoid the flap requirement. We hypothesized that if Subjected to intentional exposure, a bioshell coating over an implant would provide enhanced wound heating. Methods: Six sheets of high density polyethylene and six sheets of 24 carat pure gold wire-mesh measuring 19 mm x 25 mm were implanted subcutaneously in an immunocompetent swine model. Half of each implant group were coated with chondrocytes (50-70 million cells/cm(3)) which were suspended in Pluronic F-127 30% hydrogel; the remaining implants without chondrocytes were used as controls. At 10 weeks postimplantation, partial implant exposure via excision of overlying skin was performed to simulate extrusion and the sites were allowed to heat secondarily. Results: All (6/6) of bioshell implants achieved wound closure after exposure by the seventh post-operative day; controls achieved closure at approximately 10 days. Bioshell neocartilage was evaluated and confirmed histologically using hematoxylin and eosin and safranin O stains. Histochemically, neocartilage approximated native cartilage with 60% glycosaminoglycans content. Conclusion: A 'proof-of-principle' tissue engineered bioshell around subcutaneous high density polyethylene and gold implants generated an elastic neocartilage coating, elicited a tow inflammatory reaction, and was associated with 30% faster wound heating. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
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