Labeling of macrophages using bacterial magnetosomes and their characterization by magnetic resonance imaging

This work investigated macrophages labeled with magnetosomes for the possible detection of inflammations by MR molecular imaging. Pure magnetosomes and macrophages containing magnetosomes were analyzed using a clinical 1.5 T MR-scanner. Relaxivities of magnetosomes and relaxation rates of cells containing magnetosomes were determined. Peritonitis was induced in two mice. T-1, T-2 and T-2* weighted images were acquired following injection of the probes. Pure magnetosomes and labeled cells showed slight effects on T-1, but strong effects on T-2 and T-2* images. Labeled macrophages were located with magnetic resonance imaging (MRI) in the colon area, thus demonstrating the feasibility of the proposed approach. (C) 2006 Published by Elsevier B.V.