期刊
JOURNAL OF CONTROLLED RELEASE
卷 118, 期 2, 页码 169-176出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2006.12.011
关键词
controlled release; corneal wound healing; epidermal growth factor; cationized gelatin hydrogel; hydrogel degradation
We designed a new ophthalmic drug-delivery system for epidermal growth factor (EGF) from the biodegradable hydrogel of cationized gelatin. We placed a cationized gelatin hydrogel (CGH) with incorporated I-125-labelled EGF in the conjunctival sac of mice and measured the residual radioactivity at different times to evaluate the in vivo profile of EGF release. Approximately 60-67% and 10- 12% of EGF applied initially remained 1 and 7 days after application, respectively; whereas EGF delivered in topically applied solution or via EGF impregnation of soft contact lenses disappeared within the first day. We also placed CGH films with 5.0 mu g of incorporated EGF on round comeal defects in rabbits to evaluate the healing process using image analysis software and to assess epithelial proliferation immunohistochemically by counting the number of Ki67-positive cells. The application of a CGH film with incorporated EGF resulted in a reduction in the epithelial defect in rabbit comeas accompanied by significantly enhanced epithelial proliferation compared with the reduction seen after the topical application of EGF solution or the placement of an EGF-fice CGH film. The controlled release of EGF from a CGH placed over a comeal epithelial defect accelerated ocular surface wound healing. (c) 2007 Elsevier B.V All rights reserved.
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